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grna cas9 dual expression vector px458  (Addgene inc)


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    Structured Review

    Addgene inc grna cas9 dual expression vector px458
    Grna Cas9 Dual Expression Vector Px458, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+cas9+dual+expression+vector+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm38755139-355-21-26
    Average 96 stars, based on 4125 article reviews
    grna cas9 dual expression vector px458 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity
    Article Snippet: .. The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbook TM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138 [ https://www.addgene.org/48138/ ]). ..

    Article Title: Sequential Glycosylations at the Multibasic Cleavage Site of SARS-CoV-2 Spike Protein Regulate Viral Activation, Assembly, and Infection
    Article Snippet: .. The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbookTM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138). ..

    Article Title: Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity.
    Article Snippet: .. Genetic knock-out of GALNT7 in HEK293T The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbookTM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138 [https://www.addgene.org/48138/ ]). ..

    Expressing:

    Article Title: Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity
    Article Snippet: .. The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbook TM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138 [ https://www.addgene.org/48138/ ]). ..

    Article Title: Sequential Glycosylations at the Multibasic Cleavage Site of SARS-CoV-2 Spike Protein Regulate Viral Activation, Assembly, and Infection
    Article Snippet: .. The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbookTM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138). ..

    Article Title: Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity.
    Article Snippet: .. Genetic knock-out of GALNT7 in HEK293T The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbookTM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138 [https://www.addgene.org/48138/ ]). ..

    Plasmid Preparation:

    Article Title: Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity
    Article Snippet: .. The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbook TM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138 [ https://www.addgene.org/48138/ ]). ..

    Article Title: Sequential Glycosylations at the Multibasic Cleavage Site of SARS-CoV-2 Spike Protein Regulate Viral Activation, Assembly, and Infection
    Article Snippet: .. The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbookTM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138). ..

    Article Title: Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity.
    Article Snippet: .. Genetic knock-out of GALNT7 in HEK293T The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbookTM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138 [https://www.addgene.org/48138/ ]). ..

    Knock-Out:

    Article Title: Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity.
    Article Snippet: .. Genetic knock-out of GALNT7 in HEK293T The gRNAs targeting exon 6 of GALNT7 were designed using gUIDEbookTM and cloned into the gRNA/Cas9 dual expression vector pX458 (Addgene plasmid no.48138 [https://www.addgene.org/48138/ ]). ..



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    Addgene inc grna cas9 dual expression vector px458
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    Addgene inc grna cas9 dual expression vector pspcas9 bb 2a gfp px458
    The knockout of integrated HBV S gene by <t>CRISPR/Cas9</t> system in PLC/PRF/5 cells. (A) The plasmid pBB4.5-HBV1.2 (0.5 μg) was co-transfected with gRNA2+3, gRNA3+7 and gRNA2+3+7 expression vectors (each 0.75 μg) to PLC/PRF/5 cells. Cellular DNA was extracted at 72 h post transfection, and PCR amplifications were performed using the primers beyond the cleavage sites of each dual gRNAs. (B) The off-target effect of sgRNA was detected by T7 endonuclease I assay. Each sgRNA detected the two most likely off-target sites. (C–D) Sequencing analysis of the smaller fragment formed by sgRNA3+7 and sgRNA2+3. (E) . HBsAg level in culture supernatant was measured by using an enzyme-linked immune sorbent assay. Data are shown as mean ± SE of 3 independent samples. Statistical method: t test (two-side), * means p < 0.05, ** means p < 0.01,*** means p < 0.001. (F) The cell proliferation of PLC/PRF/5 cells after transfecting different sgRNA plasmids. Cells transfected with empty <t>PX458</t> plasmid were used as control group. Data are shown as mean ± SE of 3 independent samples. Statistical method: t test (two-side).
    Grna Cas9 Dual Expression Vector Pspcas9 Bb 2a Gfp Px458, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
    grna cas9 dual expression vector pspcas9 bb 2a gfp px458 - by Bioz Stars, 2026-11
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    Image Search Results


    The knockout of integrated HBV S gene by CRISPR/Cas9 system in PLC/PRF/5 cells. (A) The plasmid pBB4.5-HBV1.2 (0.5 μg) was co-transfected with gRNA2+3, gRNA3+7 and gRNA2+3+7 expression vectors (each 0.75 μg) to PLC/PRF/5 cells. Cellular DNA was extracted at 72 h post transfection, and PCR amplifications were performed using the primers beyond the cleavage sites of each dual gRNAs. (B) The off-target effect of sgRNA was detected by T7 endonuclease I assay. Each sgRNA detected the two most likely off-target sites. (C–D) Sequencing analysis of the smaller fragment formed by sgRNA3+7 and sgRNA2+3. (E) . HBsAg level in culture supernatant was measured by using an enzyme-linked immune sorbent assay. Data are shown as mean ± SE of 3 independent samples. Statistical method: t test (two-side), * means p < 0.05, ** means p < 0.01,*** means p < 0.001. (F) The cell proliferation of PLC/PRF/5 cells after transfecting different sgRNA plasmids. Cells transfected with empty PX458 plasmid were used as control group. Data are shown as mean ± SE of 3 independent samples. Statistical method: t test (two-side).

    Journal: Frontiers in Molecular Biosciences

    Article Title: Pacbio Sequencing of PLC/PRF/5 Cell Line and Clearance of HBV Integration Through CRISPR/Cas-9 System

    doi: 10.3389/fmolb.2021.676957

    Figure Lengend Snippet: The knockout of integrated HBV S gene by CRISPR/Cas9 system in PLC/PRF/5 cells. (A) The plasmid pBB4.5-HBV1.2 (0.5 μg) was co-transfected with gRNA2+3, gRNA3+7 and gRNA2+3+7 expression vectors (each 0.75 μg) to PLC/PRF/5 cells. Cellular DNA was extracted at 72 h post transfection, and PCR amplifications were performed using the primers beyond the cleavage sites of each dual gRNAs. (B) The off-target effect of sgRNA was detected by T7 endonuclease I assay. Each sgRNA detected the two most likely off-target sites. (C–D) Sequencing analysis of the smaller fragment formed by sgRNA3+7 and sgRNA2+3. (E) . HBsAg level in culture supernatant was measured by using an enzyme-linked immune sorbent assay. Data are shown as mean ± SE of 3 independent samples. Statistical method: t test (two-side), * means p < 0.05, ** means p < 0.01,*** means p < 0.001. (F) The cell proliferation of PLC/PRF/5 cells after transfecting different sgRNA plasmids. Cells transfected with empty PX458 plasmid were used as control group. Data are shown as mean ± SE of 3 independent samples. Statistical method: t test (two-side).

    Article Snippet: The gRNA/Cas9 dual expression vector pSpCas9(BB)-2A-GFP (PX458) was obtained from Addgene (Cambridge, MA).

    Techniques: Knock-Out, CRISPR, Plasmid Preparation, Transfection, Expressing, T7EI Assay, Sequencing, Control